Structural and Thermodynamic Characterization of Vibrio fischeri CcdB
نویسندگان
چکیده
منابع مشابه
Structural and thermodynamic characterization of Vibrio fischeri CcdB.
CcdB(Vfi) from Vibrio fischeri is a member of the CcdB family of toxins that poison covalent gyrase-DNA complexes. In solution CcdB(Vfi) is a dimer that unfolds to the corresponding monomeric components in a two-state fashion. In the unfolded state, the monomer retains a partial secondary structure. This observation correlates well with the crystal and NMR structures of the protein, which show ...
متن کاملIn silico structural analysis of quorum sensing genes in Vibrio fischeri
Quorum sensing controls the luminescence of Vibrio fischeri through the transcriptional activator LuxR and the specific autoinducer signal produced by luxI. Amino acid sequences of these two genes were analyzed using bioinformatics tools. LuxI consists of 193 amino acids and appears to contain five α-helices and six ß-sheets when analyzed by SSpro8. LuxI belongs to the autoinducer synthetase fa...
متن کاملCharacterization of pES213, a small mobilizable plasmid from Vibrio fischeri.
Most Vibrio fischeri strains isolated from the Euprymna scolopes light organ carry plasmids, often including both a large (>40kb) plasmid, and one or more small (<12kb) plasmids. The large plasmids share homology with pES100, which is the lone plasmid in V. fischeri type strain ES114. pES100 appears to encode a conjugative system similar to that on plasmid R721. The small plasmids lack extensiv...
متن کاملIn silico Prediction and Docking of Tertiary Structure of LuxI, an Inducer Synthase of Vibrio fischeri
Background: LuxI is a component of the quorum sensing signaling pathway in Vibrio fischeri responsible for the inducer synthesis that is essential for bioluminescence. Methods: Homology modeling of LuxI was carried out using Phyre2 and refined with the GalaxyWEB server. Five models were generated and evaluated by ERRAT, ANOLEA, QMEAN6, and Procheck. Results: Five refined models were gener...
متن کاملin silico structural analysis of quorum sensing genes in vibrio fischeri
quorum sensing controls the luminescence of vibrio fischeri through the transcriptional activator luxr and the specific autoinducer signal produced by luxi. amino acid sequences of these two genes were analyzed using bioinformatics tools. luxi consists of 193 amino acids and appears to contain five α-helices and six ß-sheets when analyzed by sspro8. luxi belongs to the autoinducer synthetase fa...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2010
ISSN: 0021-9258
DOI: 10.1074/jbc.m109.068429